polyclonal α lamp1 (Cell Signaling Technology Inc)
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Structured Review
Cell Signaling Technology Inc
polyclonal α lamp1
Polyclonal α Lamp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 974 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+%CE%B1+lamp1/LAMP1+XP+Rabbit+mAb/pm40184468-377-43-59
Average 97 stars, based on 974 article reviews
Polyclonal α Lamp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 974 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+%CE%B1+lamp1/LAMP1+XP+Rabbit+mAb/pm40184468-377-43-59
Average 97 stars, based on 974 article reviews
polyclonal α lamp1 - by Bioz Stars,
2026-10
97/100 stars
Images
1) Product Images from "Retromer promotes the lysosomal turnover of mtDNA."
Article Title: Retromer promotes the lysosomal turnover of mtDNA.
Journal: Science advances
doi: 10.1126/sciadv.adr6415
Figure Legend Snippet: Fig. 6. The small GTPase RAB10 promotes mitochondrial fragmentation and mtDNA degradation in lysosomes. (A) Immunostaining of HeLa cells expressing the constitutive active protein RAB10Q68L-GFP labeled with α-VPS35. (B) Manders’ correlation coefficient between RAB10 and VPS35. (C and D) Confocal images of cells ex- pressing WT RAB10-GFP, constitutive active RAB10Q68L-GFP, dominant negative RAB10T23N-GFP, in the steady state, and (D) expressing TWNKK319E-mCherry, labeled with α-TOM20. (E) Quantification of the mitochondrial morphology in RAB10 expressing cells (n = 3, >20 cells per replicate). (F and G) Cells expressing RAB10Q68L-GFP and (G) TWNKK319E-mCherry labeled with α-LAMP1 and α-dsDNA. Arrows depict RAB10-LAMP1-dsDNA foci. (H) Manders’ correlation coefficient between RAB10-GFP and LAMP1 and LAMP1 and dsDNA (n = 3, 10 images per replicate). (I) RAB10-GFP coimmunoprecipitation in the steady state and cells expressing TWNKK319E-mCherry with the lysosomal protein LAMP1. P values were calculated using one-way ANOVA with Tukey correction for multiple comparisons. Scale bars, 10 μm. Data are presented as means ± SEM.
Techniques Used: Immunostaining, Expressing, Labeling, Dominant Negative Mutation
Related Articles
Immunofluorescence:Article Title: Lysosomal uptake of mtDNA mitigates heteroplasmy Article Snippet: .. Antibodies used for immunofluorescence were: polyclonal α-TOM20 (11802-1-AP; 1:1000), α-Calnexin (10427-2-AP; 1:500) from Proteintech; monoclonal α-dsDNA (ab27156; 1:1000), α-PDHX (ab110333; 1:500), α-ATP5A (ab14748; 1:500) and polyclonal α-RAB5 (1:500) from Abcam; Article Title: Retromer promotes the lysosomal turnover of mtDNA Article Snippet: .. Antibodies used for immunofluorescence were as follows: polyclonal α-TOM20 (11802-1-AP; 1:1000) and α-VPS26A (12804-1-AP; 1:1000) from Proteintech; monoclonal α-dsDNA (ab27156; 1:1000), α-PDHX (ab110333; 1:500), α-ATP5A (ab14748; 1:500), and polyclonal α-RAB5 (1:500) from Abcam; Article Title: Retromer promotes the lysosomal turnover of mtDNA. Article Snippet: .. Antibodies used for immunofluorescence were as follows: polyclonal α- TOM20 (11802- 1- AP; 1:1000) and α- VPS26A (12804- 1- AP; 1:1000) from Proteintech; monoclonal α- dsDNA (ab27156; 1:1000), α- PDHX (ab110333; 1:500), α- ATP5A (ab14748; 1:500), and polyclonal α- RAB5 (1:500) from Abcam; Western Blot:Article Title: Retromer promotes the lysosomal turnover of mtDNA. Article Snippet: .. Antibodies used for Western blot were as follows: polyclonal α- SDHB (10620- 1- AP; 1:1000), α- TOM20 (11802- 1- AP; 1:1000), α- optineurin (10837- 1- AP; 1:1000) α- LC3B (14600- 1- AP; 1:1000), αGFP (50430- 2- AP; 1:1000), α- VPS26A (12804- 1- AP; 1:1000), α- Miro1 (#84055- 5- RR; 1:1000), and monoclonal α- GAPDH (60004- 1- Ig; 1:4000), α- GFP (66002- 1- Ig; 1:1000), and α- p62 (66184- 1- Ig; 1:1000) from Proteintech; polyclonal α- RAB5 (ab109534; 1:1000), α- VPS29 (ab236796; 1:1000), and monoclonal α- ATP5A (ab14748; 1:1000) from Abcam; Article Title: Retromer promotes the lysosomal turnover of mtDNA Article Snippet: .. Antibodies used for Western blot were as follows: polyclonal α-SDHB (10620-1-AP; 1:1000), α-TOM20 (11802-1-AP; 1:1000), α-optineurin (10837-1-AP; 1:1000) α-LC3B (14600-1-AP; 1:1000), α-GFP (50430-2-AP; 1:1000), α-VPS26A (12804-1-AP; 1:1000), α-Miro1 (#84055-5-RR; 1:1000), and monoclonal α-GAPDH (60004-1-Ig; 1:4000), α-GFP (66002-1-Ig; 1:1000), and α-p62 (66184-1-Ig; 1:1000) from Proteintech; polyclonal α-RAB5 (ab109534; 1:1000), α-VPS29 (ab236796; 1:1000), and monoclonal α-ATP5A (ab14748; 1:1000) from Abcam; Article Title: Lysosomal uptake of mtDNA mitigates heteroplasmy Article Snippet: Protein concentration was quantified using Bradford reagent (Bio-Rad, # 5000001), and equal amounts of protein were loaded in SDS-PAGE, transferred to PVDF membrane (XYZ), blocked in TBST-5% Fat-free milk, and blotted with the indicated antibodies. .. Antibodies used for western blot were: polyclonal α-SDHB (10620-1-AP; 1:1000), α-optineurin (10837-1-AP; 1:1000) α-LC3B (14600-1-AP; 1:1000), α-GFP (50430-2-AP; 1:1000) and monoclonal α-GAPDH (60004-1-Ig; 1:4000), α-GFP (66002-1-Ig; 1:1000) and α-p62 (66184-1-Ig; 1:1000) from Proteintech; polyclonal α-SAMM50 (ab133709; 1:1000), α-RAB5 (ab109534; 1:1000) and monoclonal α-ATP5A (ab14748; 1:1000) from Abcam; |